Seahorse xf24 manual

 

 

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For use with Seahorse XF24 Analyzer model number series: 100736, 100737. Assay Kits and Reagents. Part #. Description. Description XF24 V7 PS Cell Culture Microplates XF24 V28 PS Cell Culture Microplates XF24 Islet Capture Microplates. The Seahorse XF Cell Energy Phenotype Test Report Generator supports assay results from all Seahorse XF Analyzers and provides a simple, standardized Summary Report displaying the Energy Phenotype for each group or condition analyzed in a just a few short steps. Seahorse XF Analyzers utilize patented transient microchambers which enable sensitive, precise, and label-free metabolic measurements of live cells in real time. Measurement Cycle Definition. The integrated drug delivery system allows for sequential addition of up to four compounds* per well at Loading the Agilent Seahorse XFe24/XF24 Sensor Cartridge with compounds. Cell Characterization using the Seahorse XF Cell Energy Phenotype Test. Application. Obesity/Diabetes Cancer Biology Immunology Neurodegeneration Other pathways involve changes of glycolysis and mitochondria Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples. Dr. David Ferrick explains the XF Transient Microchamber.Seahorses patented Transient Microchamber is the key to the novel XF assay. The Transient The Seahorse Extracellular Flux XF24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in assessing mitochondrial function. One of the most important steps in the analysis of mitochondrial respiration The Seahorse XF24 instrument is a sensitive, high-throughput (24-well) easy-to-use technology to measure changes in O2 tension and proton concentration in the medium, as a function of time (~10 second resolution). The typical application is to interrogate mitochondrial bioenergetics in adherent The Seahorse Extracellular Flux XF24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in assessing mitochondrial function. 744-2780 [email protected] Technical Assistance: Donghai Zheng Exercise and Sport Science Brody Room 3E-57 Phone: 744-3465 [email protected] Purpose: Seahorse XF24 Extracellular Flux a period of minutes, hours or even days Re-use cell samples after completing an XF measurement Back to top. Seahorse XFe24 Analyzers is version 2.6.1 For Research Use Only. Not for use in diagnostic The Agilent Seahorse XF Real-Time ATP rate assay report generator is the data analysis software for It therefore, simplifies your data analysis and interpretation and eliminates manual, error-prone analysis

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